Microbial ecologists have investigated roles of species richness and diversity in a wide variety of ecosystems. Recently, metagenomics have been developed to measure functions in ecosystems, but this approach is cost-intensive.
We developed a novel method for the rapid and efficient reconstruction of a virtual metagenome in environmental microbial communities without using large-scale genomic sequencing [Okuda, S. et al. Nat. Commun. 3:1203 2013].
Our method is useful for 16S rRNA gene sequence data obtained from gel-based method like denaturing gradient gel electrophoresis (DGGE) analysis or large scale sequencing technologies, mapped to fully sequenced genomes, to reconstruct virtual metagenome-like organizations.
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